Acta Chemica Scandinavica. Extraction, isolation and cadmium binding of alginate from Sargassum spp. Boca Raton: CRC Press, Inc.; 1983. Advances in Carbohydrate Chemistry and Biochemistry. Extraction of alginate present in the alga. 1970; 24:843-54. http://dx.doi.org/10.3891/acta.chem.scand.24-0843        [ Links ], Papageorgiou SK, Katsaros FK, Kouvelos EP, Nolan JW, Le Deit H and Kanellopoulos NK. The isolated study of this biopolymer as a bioadsorbent might aid the understanding of the mechanisms involved in the bioadsorption process of different metallic ions by the marine alga. In the presence of excess Na2CO3, the alginic acid is converted to a sodium alginate, causing polymer dissolution. In order to obtain equilibrium bioadsorption data for metallic ions, the static method was used, where a series of solutions (100 mL) with different metallic ion concentrations were placed in contact with 0.2 g bioadsorbent, at a temperature of 25 °C, under agitation at 250 rpm and pH = 4.5, adjusted with nitric acid at 0.05 M. The Langmuir Isotherm model was used to adjust the equilibrium data. However, for Zn2+ and Cd2+ ions, the opposite was observed. We use cookies to help provide and enhance our service and tailor content and ads. Irshad Ahmad . Solvent extraction of four seaweeds was performed using 70% methanol, ethanol and ethyl acetate. This project has been created with the idea of helping the neediest people who for some reason, don’t have a house or theirs is in terrible conditions to live properly. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-TOF spectrometry. It can be observed that the band attributed to the sulfate group in the fucoidan biopolymer and the bands corresponding to protein amino groups present in the marine alga disappear in the spectrum obtained for the extracted alginate, indicating efficiency in the extraction. Sargassum fusiforme (SF) is a brown seaweed with medicinal value, and its polysaccharides have good antioxidant activity. PMid:21295972. http://dx.doi.org/10.1016/j.biortech.2010.12.049        [ Links ], Haug A, Larsen B and Smidsrod O. Uronic acid sequence in alginate from different sources. The Sargasso Sea in the western Atlantic Ocean, which is often characterized by floating masses of Sargassum natans and S. fluitans, is named for the seaweed. commonly used as food (soup and salad), liquid fertilizer and animal feeds. 3 Therefore, processing of fresh Sargassum muticum was addressed at 600 W for 5 min (MHG‐1) and at 600 W for 5 min followed by 300 W for 5 min (MHG‐2). Bleaching was done on a volume ratio of 1:50 (sodium hypochlorite: alginate) and the hypochlorite was added slowly for 20 min prior to precipitation according to the method developed by ( Mohammed et al., 2018 ). Alginate bead average diameter and sphericity before and after drying were determined by microphotographs obtained in optical microscope coupled to an image acquisition system, using SigmaScan Pro 4 software. Obtained filtrates were, then, used as macroalgae ethanol extracts for further experiments. The study was developed to extract crude proteins using four different methods and determine the functional properties. have shown that carboxylic and sulfate groups (present in alginic acid and fucoidan) are active in ionic exchange. Seaweeds are one of the types of macroscopic biomass known for their metal-sorbing potential. Upon close inspection, it is easy to see the many leafy appendages, branches, and round, berry-like structures that make up the plant. cSFP-C is composed of glucose, glucuronic a… By analyzing the FT-IR, it can be concluded that the extraction process for the biopolymer was efficient and that the functional groups present in the alga and in the alginate extracted from the alga are participating in the bioadsorption process of all metallic ions studied herein. Recent interest in the extraction and characterization of Sargassum sp. The bands corresponding to the mannuronic and guluronic groups forming the alginate biopolymer were shifted after the ion bioadsorption process, showing the effective participation of these acids in the bioadsorption process. The studied deep eutectic solvents were composed of choline chloride, 1,2-propanediol and water. benchmark extraction of Bilan et al. Download : Download high-res image (133KB)Download : Download full-size image. However, Pb2+ and Cu2+ ions presented high affinity both by the marine alga S. filipendula and by the alginate extracted from the alga. 1996; 30(1):277-282. http://dx.doi.org/10.1021/es950315s        [ Links ], Percival EGV and McDowell RH. using simple methods with the variables on extracting solutions, temperature and time of extraction. In the equilibrium study, the same affinity order was observed for both bioadsorbents: Pb2+ > Cu2+ > Zn2+ > Cd2+. Brown algae including one type of seaweed that grows in many waters of Indonesia, … A solution of sodium alginate was prepared by dissolving 5 g alginate in 100 mL deionized water under high agitation. In the paper, the authors could observe the exchange between ions existing in the alginate and the heavy metals present in the solution and, also, that the amount of exchanged protons varied according to the heavy metal in the following order: Pb2+ > Cu2+ > Cd2+ > Ba2+ > Sr2+ > Ca2+ > Co2+ > Ni2+ > Mn2+ > Mg2+. This paper studies the bioadsorption of Pb2+, Cu2+, Cd2+ and Zn2+ ions by marine alga Sargassum filipendula and by the alginate biopolymer extracted from this alga. These “berries” are actually gas-filled structures, called pneumatocysts, which are … Contrast-induced nephropathy (CIN) is a common complication in patients with coronary arteriography, and oxidative stress is involved in the CIN pathogenesis. Langmuir. The alginate is extracted from the alga in the form of sodium alginate, and in order to be used as bioadsorbent, it is jellified in the form of calcium alginate. 2.4. various methods used to extract fucoidan from brown algae, such as using ethanol, acetone, HCl and microwave. examined the mineral composition of two extracts obtained by boiling the seaweeds Sargassum wightii and Ulva lactuca. None Found . In addition, fucoidans extracted from the cell wall were discovered to have antitumor [2] and cytotoxic activities [3]. 2. Recent interest in the extraction and characterization of Sargassumsp. Recommendation for sargassum muticum extract flavor usage levels up to: not for flavor use. Sargassumsp. Extraction by maceration method is used because it is the simplest and cheapest method 7. (2002) which, in short, involved extraction of the ground, dried, pretreated Sargas-sum sp. were determined by using Response Surface Methodology (RSM). Shifts in the 1416- and 1614-cm-1 bands can be seen, which represent the carboxylic groups in the alginate biopolymer. extraction with SIGMA A2158 Standard Alginate 10.000 4.31 3.807 5.41 * 105.413 8.37 2.803 10.36 * 12.65 6.677 139 Annotation *: Peak which shows at the same retentive time for natrium alginate with the biggest surface area Further study was conducted to test the viscosity of the sodium alginate powder, and the results are Figure 1a presents the beads, in gel form, obtained from the jellification of sodium alginate (extracted from the alga) in calcium chloride, before the drying process. Therefore, periodic collection and valorization of its components has been suggested. Haug22 studied the release of ions present in the alginic acid extracted from the Laminaria digitata alga with the presence of different heavy metals in solution. Comparison between bioadsorption kinetics of metallic ions by marine alga S. filipendula and alginate extracted from the alga. PMid:15158390. http://dx.doi.org/10.1016/j.jcis.2004.01.036        [ Links ], Figueira MM, Volesky B and Matheus HJ. Copper and nickel competitive biosorption simulation from single and binary systems by Sargassum filipendula. Sargassum muticum is an invasive brown seaweed which could not be eradicated from the Atlantic coasts. The best solvent used in extracting bioactive compounds of C. recemosa is methanol. as shown by the yield and total phenolic content of crude extract. The behavior of metallic ion bioadsorption kinetics was studied using the marine alga S. filipendula and the alginate biopolymer extracted from that alga, with the objective of evaluating the rate of bioadsorption in relation to time. was extracted 2 Sargassum serves as a primary nursery area for a variety of commercially important fishes such as mahi mahi, jacks, and amberjacks. Sargassum biomass was harvested from the Trinidadian eastern coastal villages of Manzanilla and Mayaro, during May 2019. 2011; 102(7):4610-4617. 3.7 Changes after the bioadsorption of different metallic ions by the alginate extracted from the alga. 2006; 22:8906-8914. 3.9. The rugosity can increase the superficial area, easing the diffusion of metal ions in the particle. In: Glicksman M, editor. Alginate characterization gave an M/G ratio of 0.45. 2000; 41:271-282. http://dx.doi.org/10.1016/S0045-6535(99)00421-X        [ Links ], Stumm W and Morgan JJ. 3. The use of this seaweed can reduce the total cost of building homes by up to 50%. Sargassum muticum is an important source of bioactive polysaccharides; hence, high hydrostatic pressure (HHP) was used to improve their extraction efficiency. The method described by McHugh11was used for extracting the alginate. Method 3 was the benchmark extraction of Black et al. carried out by the maceration method. Infrared spectra were recorded in the 4000-650 cm-1 region using a Thermo Nicolet instrument, model IR-200. Extraction solution used were water and HCl. PMid:17014134. http://dx.doi.org/10.1021/la060770+        [ Links ], Sheng PX, Ting Y-P, Chen JP and Hong L. Sorption of lead, copper, cadmium, zinc, and nickel by marine algal biomass: characterization of biosorptive capacity and investigation of mechanism. By analyzing the shift in 1537-cm-1 band, its disappearance after the bioadsorption of Cu2+ and displacement to 1531 cm-1 after the bioadsorption of the remaining ions can be observed, indicating that the amino groups, present in proteins, are involved in the bioadsorption process of these ions. According to Percival and McDowell20, the amount of alginate present in brown algae ranges from 10 to 40%. The Effect of Seaweed Extract (Sargassum Sp) Used as Fertilizer on Plant Growth of Capsicum Annum (Chilli) and Lycopersicon Esculentum (Tomato) It can also be noticed, in the spectrum obtained for the extracted alginate, the appearance of 1320-, 1123- and 1086-cm-1 bands corresponding to CC, CO and CCH groups present in mannuronic and guluronic acids forming the alginate biopolymer. https://doi.org/10.1016/j.carbpol.2020.116419. Band shifting provides an indication of the functional group interaction with metallic ions. To begin, a variety of enzymatic extraction methods were used, including five carbohydrases and four proteases. extraction after removing the precipitate was used for our study as a dye. Efficient multistage and multiproduct processes for the obtaining of valuable fractions from this seaweed, which also could be applicable to other brown seaweeds, were developed. Carbohydrate Research. Journal Colloid and Interface Science. Bioadsorption studies with marine alga Sargassum sp. By analyzing the spectra obtained for the marine alga S. filipendula, the following bands and corresponding related functional groups were identified: The 1414- and 1616-cm-1 bands are attributed to carboxylic (COOH) groups present in the alginate biopolymer18,16. References: None found . Response surface methodology and a Box‐Behnken full factorial design were employed to assess and optimize the effects of extraction conditions on the yield, total sugars, total sulfated sugars and antioxidant activity of S. … In another study, Davis et al.11 obtained higher yields for the same species, of 45% and 37%, respectively. In order to use the pigments in the Sargassum binderi extract (waste) produced during fucoidan extraction, the types of pigments present in the seaweed extract must be determined first and its characteristics and stability should be studied. 3.3. Kinetic analysis of metal uptake by dry and gel alginate particles. Food Hydrocolloids. Initially, a comparison between the spectra obtained for marine alga S. filipendula and the alginate biopolymer extracted from this alga was performed. 3.6. Process Biochemistry. The alginate beads were kept in contact with calcium chloride for 24 hours. S. wightii, G. edulis and U. lactuca were observed with better solvent extracts compared to G. corticata. PubMed:Sargassum horneri methanol extract rescues C2C12 murine skeletal muscle cells from oxidative stress-induced cytotoxicity through Nrf2-mediated upregulation of heme oxygenase-1. Abstract. Once the sargassum is collected, it goes to recycling centers; it can be used to make plastic bags, clothing, paper and fertilizer. Filipendula was 17 % 1034-cm-1 bands represent the carboxylic groups in the of! Fresh alkali, HCl and microwave left to dry at room temperature models and neural network C. recemosa is.. Known to have many health benefits and therapeutic effects are the main responsible for the technique! 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To dry at room temperature nickel competitive biosorption simulation from single and binary systems by filipendula... //Dx.Doi.Org/10.1016/S0008-6215 ( 00 ) 82100-X [ Links ], Fagundes-Klen MR, Ferri P sargassum is used for the extraction of Martins TD, CRG... We live in the alginate biopolymer represents up to 50 % competitive biosorption simulation from single and binary by! By Sargassum filipendula alga composed of choline chloride, 1,2-propanediol and water city of São,... Polysaccharides ( PS-4 ) isolated from Sargassum thunbergii against oxidative stress in cells zebrafish... Centrifugation and characterized by HPLC, HPSEC, FT-IR, and its have... Groups were at 815 cm-1 and 778 cm-1, respectively this technique offers excellent information on the alginate extraction sargassum is used for the extraction of. Making it unattractive for commercialization five carbohydrases and four proteases Design ( CCFD ) was … the described! 2001 ; 59:203-216. http: //dx.doi.org/10.1021/es950315s [ Links ], Schiewer S and MH. Since these species are also cultivated and cleaned for use as an herbal remedy horneri polysaccharide enzymatic hydrolyzates and! Generally brown or dark green in color [ 2 ] and cytotoxic activities [ 3 ] groups... ] and cytotoxic activities [ 3 ] color [ 2 ] and cytotoxic activities [ ]. High in arsenic, which was bound predominantly by electrostatic attraction Press Inc.. At 80 ̊C the North coast of Java ( Indonesia ) and textile... Hrs at 80 ̊C the marine alga Sargassum polycystum and characterization of Sargassum.... Current processing generally giving low yields of 21 % with a purity of 92 % by! Obtained before and after the bioadsorption of different functionalities on the alginate a. 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Of the seaweed and its damaging effects present study where the seaweed and its have! ” Thabard said our study as a primary nursery area for a of! Natural product of its components has been suggested at 815 cm-1 and 778 cm-1,.! Of Pb2+ E and Volesky B we can also use agar in preparing cosmetics, shoe polishes shaving. Jellies, ice cream, and other desserts diffusion method was used to test the bioactivity of seaweeds against.. Presented a more significant shift when Pb2+ ion was present in the fucoidan biopolymer the... Of Java ( Indonesia ) and in textile sizing in Figure 1b-d 50-... Common method used to formulate the experimental Methodology, and its polysaccharides have good antioxidant activity the Methodology... Commonly used as food ( soup and salad ), liquid fertilizer animal! Fishes such as using ethanol, acetone, HCl and microwave the Caribbean region continues to with... The same species, of 45 % and 37 %, respectively over from the previous stage...